Differentiation of Xylella fastidiosa strains via analysis of environmentally-mediated genes

Attempts so far to elucidate the genetic relationships between strains of Xylella fastidiosa (XF) have shown that genotypes tend to cluster into groups based on the host plant species from which they were isolated. In this project we are investigating the genetic variation of XF isolated from the same host in different geographic areas of the US. Sequence analysis of environmentally-mediated genes (genes thought to be influenced by environmental factors) is being applied to identify strain relationships. Multi-locus sequence analysis (MLSA) is being used for genes related to processes important for establishing XF infections such as surface attachment, biofilm formation, virulence, and nutrient transport and utilization. These types of genes may be more relevant to host-based genetic variability.

X. fastidiosa isolate WM1-1 from a Mourvedre grapevine in Dahlonega, Georgia

X. fastidiosa isolate F2-3 from a Vidal grapevine in Dahlonega, Georgia