Joseph W. Kloepper Professor 303 Life Sciences Building Auburn University, Auburn, Alabama 36849 Phone: 334 844-1950 Fax: 334 844-5067 E-Mail: jkloeppe@acesag.auburn.edu PGPR NEWSLETTER:http://www.ag.auburn.edu/pgpr Bacterial Strain-Identification and Mutant Analysis Service (BSI-MAS):http://www.ag.auburn.edu/dept/ent/services/bacterid.htm

	RESEARCH AREAS: Research has been directed at basic and applied aspects of using beneficial bacteria as microbial inoculants to promote plant growth and provide biological disease control. This work has emphasized PGPR (plant growth-promoting rhizobacteria) and endophytic bacteria. Fields of investigation include microbial ecology, plant and microbial physiology, effects on several pathogens, delivery systems, and mechanisms of biological control. Greenhouse and field evaluations are conducted annually to provide biological support for more basic investigations. Crop systems used currently in this work include cotton, tomato, and cucumber. Pathogens used in these studies include Pythium ultimum, Rhizoctonia solani, Fusarium oxysporum, Pseudomonas syringae pv. lachrymans, Colletotrichum orbiculare, Erwinia tracheiphila, and cucumber mosaic virus. Mechanism of action work includes transcriptional and translational changes in plants.
	EDUCATION: 1980 Ph.D., Plant Pathology, University of California, Berkeley. Ph.D. Thesis: The Role of Rhizobacteria in Increasing Plant Growth and Yield. M.N. Schroth, Major Professor. 1977 M.S., Plant Pathology, Colorado State University 1975 B.S., Botany and Plant Pathology, Colorado State University
	PROFESSIONAL EXPERIENCE: 1992-Present Professor, Department of Plant Pathology, Auburn University 1993-1998 Head, Department of Plant Pathology, Auburn University 1998-Present Director, Biological Control Institute, Auburn University 1992 Acting Department Head, Department of Plant Pathology, Auburn University 1992-1993 Director, Biological Control Institute, Auburn University 1989-1992 Associate Professor, Department of Plant Pathology, Auburn University 1987-1989 Manager, Microbial Inoculants Allelix Agriculture, Mississauga, Ontario 1985-1987 Group Leader, Agricultural Microbiology, Allelix Agriculture 1983-1985 Senior Research Scientist, Allelix Inc., Mississauga, Ontario 1982-1983 Research Scientist, Advanced Genetic Sciences, Inc., Manhattan, Kansas 1979-1982 Assistant Professor, Plant Pathology, University of California, Berkeley
	UNIVERSITY TEACHING: Courses PLPA 616: Plant/Bacterial Interactions PLPA 719: Grant Writing PLPA 619: Biological Control
	MAJOR PROFESSOR FOR:           Student Degree Year Graduated Martinez, Natalia Ph.D. 2000 Adams, Pam Ph.D. 2000 Rivas, Maria M.S. 1999 Raupach, Georg Ph.D. 1998 Press, Caroline Ph.D. 1997 Jetiyanon, Kanchalee Ph.D. 1997 Mahaffee, Walter Ph.D. 1996 Liu, Li Ph.D. 1994 Musson, George M.S. 1993 Wei, Gang Ph.D. 1993 Press, Caroline M.S. 1992 McInroy, John M.S. 1992
	REFEREED RESEARCH PAPERS, BOOK CHAPTERS, AND REVIEWS -- 1995 TO PRESENT:  PDF-format copy available on-line.  Click on .  Requires Adobe Acrobat Reader.           Zhang, S., Reddy, M. S., Kokalis-Burelle, N., Wells, L. W. Nightengale, S. P., Kloepper, J. W. 2001. Lack of Induced Systemic Resistance in Peanut to Late Leaf Spot Disease by Plant Growth-Promoting Rhizobacteria and Chemical Elicitors. Plant Dis. 85:879-884. Press, C. M., Loper, J. E. and Kloepper, J. W. 2001. Role of Iron in Rhizobacteria-Mediated Induced Systemic Resistance of Cucumber. The American Phytopathological Society. 91: 593-598. Zehnder, G. W., Murphy, J. F., Sikora, E. J., Kloepper, J. W. 2001. Application of rhizobacteria for induced resistance. European Journal of Plant Pathology. 107: 39-50. Zehnder, G. W., Yao, C., Wei, G and Kloepper, J. W. 2000. Influence of Methyl Bromide Fumigation on Microbe-induced Resistance in Cucumber. Biocontrol Science and Technology. 10, 687-693. Raupach, G. S. and Kloepper, J. W. 2000. Biocontrol of Cucumber Diseases in the Field by Plant Growth-Promoting Rhizobacteria With and Without Methyl Bromide Fumigation. Plant Disease. 84:1073-1075. Zehnder, G. W., Yao, C., Murphy, J. F., Sikora, E., R., Kloepper, J., W. 2000. Induction of resistance in tomato against cucumber mosaic cucumvirus by plant growth promoting rhhizobacteria. Biocontrol 45: 127-137. Murphy, J. F., Zehnder, G. W., Schuster, D. J., Sikora, E. J., Polston, J.E., and Kloepper, J. W. 2000. Plant growth-promoting rhizobacterial mediated protection in tomato against Tomato Mottle Virus. Plant Disease 84: 779-784. Park, K. S. and Kloepper, J. W. 2000. Activation of PR-1a promoter by Rhizobacteria that induce systemic resistance in Tobacco against Pseudomonas syringae pv. tabaci. Biological Control 18: 2-9. Varga-Ayala, R. Rodríguez-Kábana, R., Morgan-Jones, G., McInroy, J.A., and Kloepper, J.W. 2000. Shifts in soil microflora induced by velvetbean (Mucuna deeringiana) in cropping systems to control root-knot nematodes. Biological Control 17: 11-22. Hallmann, J., Rodríguez-Kábana, R., and Kloepper, J.W. 1999. Chitin-mediated changes in bacterial communities of the soil, rhizosphere and within roots of cotton in relation to nematode control. Soil Biol. Biochem. 31:551-560. Kloepper, J.W., Rodríguez-Kábana, R., Zehnder, G.W., Murphy, J., Sikora, E., and Fernández,, C. 1999. Plant root-bacterial interactions in biological control of soilborne diseases and potential extension to systemic and foliar diseases. Australasian Plant Pathology 28:27-33. Zehnder, G. W., Yao, C., Murphy, J. F., Sikora, E. R., Kloepper, J. W., Schuster, D. J., and Polston, J. E. 1999. Microbe-Induced Resistance Against Pathogens and Herbivores: Evidence of Effectiveness in Agriculture, pp. 335-355. In: Induced Plant Defenses Against Pathogens and Herbivores: Biochemisry, Ecology and Agriculture, A. A. Agrawal, et.al. eds. APS Press, St. Paul, MN. Benhamou, N., Kloepper, J.W., and Tuzun, S. 1998. Induction of resistance against Fusarium wilt of tomato by combination of chitosan with an endophytic bacterial strain: ultrastructure and cytochemistry of the host response. Planta 204:153-168. Chabot, R., Beauchamp, C.J., Kloepper, J.W., and Antoun, H. 1998. Effect of phosphorus on root colonization and growth promotion of maize by bioluminescent mutants of phosphate solubilizing Rhizobium leguminosarum biovar phaseoli. Soil Biol. Biochem. 30:1615-1618. Enebak, S.A., Wei, G., and Kloepper, J.W. 1998. Effects of plant growth-promoting rhizobacteria on loblolly and slash pine seedlings. Forest Science. 44:139-144. Hallmann, J., Quadt-Hallmann, A., Rodríguez-Kábana, R., and Kloepper, J.W. 1998. Interactions between Meloidogyne incognita and endophytic bacteria in cotton and cucumber. Soil Biol. Biochem. 30:925-937. Raupach, G.S., and Kloepper, J.W. 1998. Mixtures of plant growth-promoting rhizobacteria enhance biological control of multiple cucumber pathogens. Phytopathology 88:1158-1164. Zehnder, G.W, Yao, C., Murphy, J. F., Sikora, E. R., Kloepper, J. W., Schuster, D. J., and Polston, J. E. 1998. Microbe-induced resistance: A novel strategy for control of insect-transmitted diseases in vegetables. In: E.B. Radcliffe and W.D. Hutchison [eds], Radcliffe's IPM World Textbook, URL: http://ipmworld.umn.edu, University of Minnesota, St. Paul, MN Bauske, E.M., Backman, P. A., Harper, K. M., Brannen, P. M., Rodríguez-Kábana, R. and Kloepper, J. W. 1997. Effect of botanical aromatic compounds and seed-surface pH on growth and colonization of cotton plant growth-promoting rhizobacteria. Biocontrol Science and Technology 7:415-421. Hallmann, J., Kloepper, J.W. , and Rodríguez-Kábana, R. 1997. Application of the Scholander pressure bomb to studies on endophytic bacteria of plants. Can J. Microbiol. 43:411-416. Hallman, J., Quadt-Hallmann, A., Mahaffee, W. F., and Kloepper, J. W. 1997. Bacterial endophytes in agricultural crops. Can.J. Microbiol. 43:895-914. Mahaffee, W.F., Bauske, E.M., van Vuurde, J.W.L, van der Wolf, J.M., van den Brink, M., and Kloepper, J.W. 1997. Comparative analysis of antibiotic resistance, immunofluorescent colony staining, and a transgenic marker (bioluminescence) for monitoring the environmental fate of a rhizobacterium. Appl. Environ. Microbiol. 63:1617-1622. Mahaffee, W.F. and Kloepper, J.W. 1997. Bacterial communities of the rhizosphere and endorhiza associated with field-grown cucumber plants inoculated with a plant growth-promoting rhizobacterium or its genetically modified derivative. Can. J. Microbiol. 43:344-353. Press, C.M., Wilson, M., Tuzun, S., Kloepper, J.W. 1997. Salicylic acid produced by Serratia marcescens 90-166 is not the primary determinant of induced systemic resistance in cucumber or tobacco. Molec. Plant-Microbe Interactions 10:761-768. Quadt-Hallmann, A., Benhamou, N., and Kloepper, J.W. 1997. Bacterial endophytes in cotton: mechanisms of entering the plant. Can. J. Microbiol. 43:577-582. Quadt-Hallmann, A., Hallmann, J., and Kloepper, J.W. 1997. Bacterial endophytes in cotton: localization and interaction with other plant-associated bacteria. Can. J. Microbiol. 43:254-259. Zehnder, G., Kloepper, J., Tuzun, S., Yao, C. Wei, G., Chambliss, O., and Shelby, R. 1997. Insect feeding on cucumber mediated by rhizobacteria-induced plant resistance. Entomologia Experimentalis et Applicata 83:81-85. Zehnder, G., Kloepper, J., Yao, C., and Wei, G. 1997. Induction of systemic resistance in cucumber against cucumber beetles (Coleoptera: Chrysomelidae) by plant growth-promoting rhizobacteria). J. Econ. Entomol. 90:391-396. Benhamou, N., Kloepper, J.W., Quadt-Hallmann, A., and Tuzun, S. 1996. Induction of defense-related ultrastructural modifications in pea root tissues inoculated with endophytic bacteria. Physiol. Plant Pathol. 112:919-929. Chabot, R., Antoun, H., Kloepper, J.W., and Beauchamp, C.J. 1996. Root colonization of maize and lettuce by bioluminescent Rhizobium leguminosarum biovar phaseoli. Appl. Environ. Microbiol. 62:2767-2772. Chen, Y., Mei, R., Lu, S., Liu, L., and Kloepper, J.W. 1996. The use of yield-increasing bacteria (YIB) as plant growth-promoting rhizobacteria in Chinese agriculture. In: Management of Soilborne Disease, C.K. Gupta and R. Utkehde, eds. Kalyani Publishers, New Delhi. Pages 165-184. Kloepper, J. W. 1996. Host specificity in microbe-microbe interactions. BioScience 46: 406-409. Kloepper, J. W., Zehnder, G. W., Tuzun, S., Murphy, J. F., Wei, G., Yao, C., and Raupach, G.. 1996. Toward agricultural implementation of PGPR-mediated induced systemic resistance against crop pests. In: W. Tang, R.J. Cook, and A. Rovira, eds. Advances in Biological Control of Plant Diseases. China Agricultural University Press, Beijing. pp. 165-174. Press, C.M., W.F. Mahaffee, J.H. Edwards, and J.W. Kloepper. 1996. Organic by-product effects on soil chemical properties and microbial communities. Compost Sci. & Utilization 4:70-80. Quadt-Hallmann, A., and Kloepper, J.W. 1996. Immunological detection and localization of a cotton endophyte, Enterobacter asburiae, strain JM22 in different plant species. Can. J. Microbiol. 42: 1144-1154. McInroy, J.A., Musson, G., Wei, G., and Kloepper, J.W. 1996. Masking of antibiotic-resistance upon recovery of endophytic bacteria. Plant and Soil 186: 213-218. Raupach, G.S., Liu, L., Murphy, J.F., Tuzun, S., and Kloepper, J.W. 1996. Induced systemic resistance in cucumber and tomato against cucumber mosaic cucumovirus using plant growth-promoting rhizobacteria (PGPR). Plant Dis. 80:891-894. Wei, G., Kloepper, J.W., and Tuzun, S. 1996. Induced systemic resistance to cucumber diseases and increased plant growth by plant growth-promoting rhizobacteria under field conditions. Phytopathology 86:221-224. Wei, G., Kloepper, J. W., and Tuzun, S. 1996. Evaluation of induced systemic resistance and plant growth promotion with mixtures of plant growth-promoting rhizobacteria strains in the field. In: W. Tang, R.J. Cook, and A. Rovira, eds. Advances in Biological Control of Plant Diseases. China Agricultural University Press, Beijing. pp. 223-226. Wilson, M., Ji, P., Campbell, H. L., and Kloepper, J. W. 1996. Development of an integrated biological control strategy for bacterial speck of tomato caused by Pseudomonas syringae pv. tomato. In: W. Tang, R.J. Cook, and A. Rovira, eds. Advances in Biological Control of Plant Diseases. China Agricultural University Press, Beijing. Chen, C., Bauske, E.M., Musson, G. Rodríguez-Kábana, R., and Kloepper, J.W. 1995. Biological control of Fusarium wilt on cotton by use of endophytic bacteria. Biological Control 5:83-91. Kloepper, J. W. 1995. Plant growth-promoting rhizobacteria (other systems). In: Azospirillum/Plant Associations. Y. Okon, ed. CRC Press, Boca Raton. pp. 137-166. Liu, L., Kloepper, J.W., and Tuzun, S. 1995. Induction of systemic resistance in cucumber by plant growth-promoting rhizobacteria: duration of protection and effect of host resistance on protection and root colonization. Phytopathology 85:1064-1068. Liu, L., Kloepper, J.W., and Tuzun, S. 1995. Induction of systemic resistance in cucumber against bacterial angular leaf spot by plant growth-promoting rhizobacteria. Phytopathology 85:843-847. Liu, L., Kloepper, J.W., and Tuzun, S. 1995. Induction of systemic resistance in cucumber against Fusarium wilt by plant growth-promoting rhizobacteria. Phytopathology 85:695-698. Mickler, C.J., Bowen, K.L., and Kloepper, J.W. 1995. Evaluation of selected geocarposphere bacteria for biological control of Aspergillus flavus in peanut. Plant and Soil 175:291-299. McInroy, J.A., and Kloepper, J.W. 1995. Population dynamics of endophytic bacteria in field-grown sweet corn and cotton. Can. J. Microbiol. 41:895-901. McInroy, J.A., and Kloepper, J.W. 1995. Survey of indigenous bacterial endophytes from cotton and sweet corn. Plant and Soil 173:337-342. Musson, G., McInroy, J.A., and Kloepper, J.W. 1995. Development of delivery systems for introducing endophytic bacteria into cotton. Biocontrol Sci & Technol. 5:407-416. Tuzun, S., and Kloepper, J.W. 1995. Practical application and implementation of induced resistance. In: Induced Resistance to Diseases in Plants. R. Hammerschmidt and J. Kuc, Eds. Kluwer Academic Publishers, Dordecht, the Netherlands. pp. 152-168. Tuzun, S., and Kloepper, J.W. 1995. Potential application of plant growth-promoting rhizobacteria to induce systemic disease resistance. In: Novel Approaches to Integrated Pest Management. R. Reuveni, Ed. Lewis Publishers, Boca Raton. pp. 115-127. Mahaffee, W.F., and Kloepper, J.W. 1994. Applications of plant growth-promoting rhizobacteria in sustainable agriculture. In: Soil Biota, Management in Sustainable Farming Systems. C.E. Pankhurst, B.M. Doube, V.V.S.R. Gupta, and P.R. Grace, Eds. CSIRO Information Services, East Melbourne, Victoria, Australia. pp. 23-31. McInroy, J.A., and Kloepper, J.W. 1994. Studies on indigenous endophytic bacteria of sweet corn and cotton. In: Molecular Ecology of Rhizosphere Microorganisms, Biotechnology and the Release of GMOs. F. O'Gara, D.N. Dowling, and B. Boesten, Eds. VCH, New York. pp. 19-27. Tuzun, S., and Kloepper, J. W. 1994. Induced systemic resistance by plant growth-promoting rhizobacteria. In: Improving Plant Productivity with Rhizosphere Bacteria. M.H. Ryder, P.M. Stephens, and G.D. Bowen, eds. CSIRO, Adelaide, South Australia, Australia. pp. 104-109. Edwards, J.H., Press, C.M., Mahaffee, W.F., and Kloepper, J.W. 1994. Direct application effects of municipal solid waste on modification of soil biota and crop production. In: Soil Biota, Management in Sustainable Farming Systems. C.E. Pankhurst, B.M. Doube, V.V.S.R. Gupta, and P.R. Grace, Eds. CSIRO Information Services, East Melbourne, Victoria, Australia. pp. 91-93.
	Photos courtesy of: J. W. Kloepper
	Updated: September 26, 2001